See GENE regulation in 3D
Chromatin organization plays a key role in gene regulation. Genome-wide association studies for complex diseases most often implicate variats in non-coding parts of the genome. Simply assuming that disease associated enhancers affect nearby genes turns out to be wrong nearly half the time. 1-4 to the decipher the mechanisms of disease, you need to identify the long-range interactions between regulatory elements.
One-dimensional (1D) epigenomic methods like ChIP-seq or ATAC-seq only give you part of the picture. HiChIP provides the complementary data needed to put 1D data in proper context. The Arima-HiChIP protocol consolidates ChIP-seq and Hi-C into one targeted assay to offer high-resolution 3D contact maps of active enhancers and their target genes.
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