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See GENE regulation in 3D
 

 Chromatin organization plays a key role in gene regulation. Genome-wide association studies for complex diseases most often implicate variats in non-coding parts of the genome. Simply assuming that disease associated enhancers affect nearby genes turns out to be wrong nearly half the time. 1-4 to the decipher the mechanisms of disease, you need to identify the long-range interactions between regulatory elements.

One-dimensional (1D) epigenomic methods like ChIP-seq or ATAC-seq only give you part of the picture. HiChIP provides the complementary data needed to put 1D data in proper context. The Arima-HiChIP protocol consolidates ChIP-seq and Hi-C into one targeted assay to offer high-resolution 3D contact maps of active enhancers and their target genes.

Arima-HiChIP protocol uncovers cell-type specific loops associated with active promoters


Reproducible discovery of active gene regulatory chromatin loops.
Chromatin looping reproducibility analysis in our H3K27 and H3K4me3 Arima-HiChIP data. The pie charts the total number of loops from one replicate that were identified (marked as "common") or not identified (marked as "differential") in the other replicate.

 

Developed & Optimized by chromatin conformation experts


 The Arima-HiChIP protocol is thge gold-standard method developed by the pioneers in chromatin conformation capture assays. This treamlined HiChIP protocol shows proven performance for accuracy and reliability -- validated by multiple customers and external collaborators.

Out multi-restriction-enzyme-based kit offers reproducibility with the genome coverage needed for high-quality data. We provide technical support throughout your experiment. Including data analysis and visualization. Leading open-source bioinformatics tools like FitHiChIP and an optimized MAPS pipeline sort out the noise to find true chromatin connections

 

Download HiChIP Application Note

Accurate and cost-effective discovery of active gene regulatory interactions

The Arima-HiChIP protocol is a reproducible and simplified protocol for identifying long-range genomic contacts mediated by proteins. HiChIP combines Hi-C and chromatin immunoprecipitation with antibodies against a protein of interest to enrich for interactions mediated by this protein. As a targeted approach, HiChIP requires less sequencing compared to Hi-C. The Arima-HiChIP assay targets H3K27ac or H3K4me using Arima-validated antibodies, which are specific for interactions linking active regulatory domains.

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Arima-HiChIP

The Arima-HiChIP protocol streamlines HiChIP to a robust and reproducible 2-day workflow.

To facilitate broad and easy access to powerful HiChIP technology, we developed an easy-to-use and reproducible Arima-HiChIP-enabled kit that streamlines the HiChIP protocol via a 2-day workflow and is optimized for efficient capture of chromatin looping (3D) and ChIP enrichment (1D) data.

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 References
  1. Hait, et al. Genome Biol. (2018) doi: 10.1186/s13059-018-1432-2
  2.  Perrealt, et al. Exp Hematol. (2017) doi: 10.1016/j.exphem.2017.03.004
  3.  Yao, et al. Crit Rev Biochem Mol Biol. (2015) doi: 10.3109/10409238.2015.1087961
  4.  Andersson, et al. Nature. (2014) 507:455-461.doi:10.1038/nature/12787
  5.  Collaborators: Ari Melnick (Weill Cornell Medical College). Lorenzo Puri (SanFord Burnham Prebys Discovery Institute), Yin Shen (University of California, San Francisco), Peter Scacheri (Case Western University School of Medicine), Tamas Ordog(Mayo Clinic)