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3D Genomics is Helping Cancer Researchers Solve Undiagnosable tumorsLearn More

Reveal the Functional and Pathological Importance of Architectural Features of the Genome

The 3D structure of the genome is known to have significant impacts on human health and disease. 3D organizational features including loops, TADs, and compartments can modify promoter-enhancer interactions, gene splicing during transcription, gene expression, and DNA replication.

The pathological implications of these alterations include chromosome translocations, the creation of oncogenic fusion transcripts, and altered regulation of distant genes caused by long-range effects of single nucleotide variants. Capturing 3D genomic information enables more complete characterization of disease mechanisms and identification and validation of novel therapeutic targets and pathways.

 

 

Why Choose Arima Hi-C for Your Research?

Proven performance for greater accuracy of chromatin loop and topological domain detection, even at reduced sequencing depths

Fast and user-friendly workflows to go from sample to discovery using either genome-wide or targeted approaches

Quality you can trust, with built-in QC steps to ensure you get reliable sequencing results every time

Support for a broad range of sample types and an optimized protocol for low sample input

High-resolution analysis of 3D chromatin isolated from patient-derived Ewing sarcoma cells revealed reprogramming of higher order chromatin interactions, changes in A/B compartments, TAD boundaries and enhancer-promoter chromatin loops.

Explore Disease Mechanisms

The 3D organization of chromatin is a critical regulator of genome function. ​​Obtain a more complete understanding of gene regulation by utilizing 3D genomic information to uncover the structural framework by which DNA chemical modifications, histone modifications, and chromatin binding proteins cooperate to orchestrate transcriptional regulation.

Develop Novel Therapeutic Approaches

Use Arima Hi-C technology to discover, validate, and apply 3D genomic signatures to identify novel diagnostic or prognostic markers, identify novel therapeutic targets, or assess therapeutic response to treatment.

Oncogenic 3D genome conformations were used to identify novel therapeutic vulnerabilities in ependymoma, demonstrating the ability of Arima Hi-C technology to reveal tumor-dependency genes and pathways by oncogenic 3D genome conformations even in tumors that lack known genetic alterations.

Lindsey Montefiori

PhD, Postdoctoral Fellow St. Jude Children’s Research Hospital
“Hi-C technologies can be challenging to work with, but the Arima-HiC+ kit easily enabled us to perform H3K27ac HiChIP in both cell lines and limiting numbers of primary samples. We readily interrogated oncogenic structural variants in leukemia patient samples, including enhancer hijacking and de novo enhancer formations.”

HiCHiP

Combine the power of Arima HiC+ with chromatin immunoprecipitation (ChiP) to discover active gene regulatory interactions.

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