Benefits of Arima-HiC+ FFPE
The Arima-HiC+ FFPE Kit and workflow are ideal for the detection and discovery of structural variants from archival or clinical samples.
Detect both coding and non-coding structural variants
Link structural variants to impacts on gene function
Efficiently generate multiple data types from a single assay
Generate Comprehensive Insights
The Arima-HiC+ FFPE kit provides dependable results from this hard-to-use sample type that are highly correlated with results from fresh-frozen tissue samples. Learn more about our structural genomics application.
FFPE Sample Results are Highly Correlated to Frozen Tissues. Matched frozen and FFPE tissue of K562 cell cultures were processed with the Arima-HiC+ and Arima-HiC+ FFPE kits, respectively. Libraries were generated following Arima protocols and sequenced using Illumina next generation sequencing (57M and 27M reads were generated, respectively). Representative data for Chromosome 2 at 500 Mb are shown in a. Hi-C contact heat map (500Mb resolution) and b. correlation plot of the normalized Hi-C counts per bin for frozen versus FFPE samples showing a high correlation (r=0.987).
Arima-HiC+ FFPE Workflow
With our easy-to-use workflow you can rapidly go from sample to discovery.
Dewaxing and Arima-HiC prep
Use 5 mm3 of tissue (~5 x 5µm FFPE tissue sections)
Pre-validated Library Prep Protocol
Using the Swift Biosciences Accel-NGS 2S Plus DNA Library Kit
Next Generation Sequencing
2 x 150 paired-end reads from Illumina
Powerful Analysis Built on Open Source Tools
Matija Snuderl, MDDirector of Molecular Pathology and Diagnostics, NYU Langone Medical Center
Explore Our Resources
Learn more about how you can use Arima HiC+ Kits and Workflows in your research: