Arima Genomics Hi-C Technology

Arima-HiC is a proximity ligation method that captures the three-dimensional organizational structure of chromatin, where genomic sequences that are distal to each other in linear distance can be closer to each other in the 3D space. The high-resolution, genome-wide map of interacting genetic loci that is generated from Hi-C data can then be used across multiple genomic applications including identification of promoter-enhancer interactions for gene regulation studies and scaffolding contigs for genome assemblies to define chromosomes de novo.

Arima-HiC vs Conventional NGS

Preserve Sequence and Structure

When using sequencing technologies alone, extracted DNA loses spatial proximity info that is critical to 3D genome organization and gene regulation. Figure 5(a) illustrates by only utilizing sequencing and not Arima-HiC, extracted DNA in step (1) loses spatial proximity info that is critical 3D genome organization information and long-range genetic information. In 5(b), Adding Arima-HiC to your sequencing workflow enables discovery of spatially proximal sequences not evident in linear sequencing.

Arima-HiC Emerging Applications

Arima-HiC is proving highly robust and highly flexible. Building off the base technology, we continue to innovate enhancements and expand applications.


Capture HiC

Hi-C

Biotin Enrichment

Library Prep

Target Enrichment

Sequencing

Detect genomic interactions at targeted gene promoters, disease associated loci, TADs, and more

HiChIP

Hi-C

ChIP

Biotin Enrichment

Library Prep

Sequencing

Detect genomic interactions mediated by one protein factor (e.g. H3K27ac)

Summary

Arima-HiC technology captures DNA sequence and structure for current and emerging applications in epigenetics and genome assembly studies. The Arima-HiC technology delivers accurate and reproducible results at reduced sequencing depth; ensures HiC sequencing success via quick, easy, and predictive experimental QC checkpoints; reduces experiment complexity with a fast and user-friendly workflow; and enables broad application with flexible input requirements.

Arima-HiC Kit