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Improve Resolution of High-Throughput Chromatin
Conformation Capture with Arima Capture-HiC

 

 

Uncover Chromatin Conformation and Epigenomic Features
Around Targeted Genes

 

The Arima-HiC+ kit is a highly simplified and robust protocol that streamlines Hi-C to a 6-hour workflow followed by library prep and next-generation sequencing. The Arima-HiC+ kit empowers researchers to leverage Hi-C technology to perform either genome-wide or targeted identification of promoter-enhancer interactions, link non-coding, disease-associated variants to target promoters, and uncover how perturbations in genome conformation impact gene regulation in disease contexts

  • Proven performance—greater accuracy of chromatin loop and topological domain detection even at reduced sequencing depths
  • Fast and user-friendly workflow—rapid 6-hour, Hi-C workflow
  • Assured quality—rapid and easy library QC steps that accurately predict library quality, ensuring sequencing success
  • Flexible input requirements—Support for a broad range of sample types and an optimized protocol for low sample input

Partnered with Agilent to Achieve Cost-Effective, Sub-Kilobase Resolution

The three-dimensional (3D) organization of chromatin can be determined through genome-wide next generation sequencing (NGS) after chromatin conformation capture (3C), a technique called Hi-C. High-resolution maps of chromatin interactions provide insight into chromatin organization and gene regulation in disease contexts. Deep sequencing and high levels of sequencing coverage are necessary to achieve the resolution necessary for chromatin studies.

Achieving the level of depth and coverage required for the whole genome (whole genome sequencing) can take a lot of time, cost, and data processing power. Arima partners with Agilent Technologies to provide a streamlined target enrichment workflow for HiC (Capture HiC). Capture HiC combines the 3C technology of Hi-C with the targeted results of hybridization capture. This targeted next generation sequencing technique uses biotinylated probes to capture, or enrich, regions of interest, saving time and money. Targeting specific regions of the genome focuses results, lowering the barrier to generating high-resolution HiC data. Read more about Utilizing Agilent SureSelect XT HS2Target Enrichment and Arima-HiC to Improve Resolution of High Throughput Chromatin Conformation Capture.


Rapid 6-hour protocol

  • Chromatin cross-linking
  • Digest crsslinked chromatin
  • Ligation
  • QC Check


Pre-validated library

  • KAPA HyperPrep
  • Swift Accel-NGS 25 Plus
  • Agilent SureSelect XT
  • Illumina TruSeq
  • NEBNext Ultra II
  • Other


Optimized for Next-generation sequencers

  • Illumina NovaSeq
  • Illumina HiSeq
  • Illumina NextSeq
  • Illumina MiSeq
  • BGI-Seq


Powerfuk analysis with open source tools

  • Arima Genomics Mapping Pipeline
  • SALSA
  • Other

Resources

 

Application Note:

The Arima-HIC kit for Signal-Enriched, High-Quality, and Reproducible Genome COnformation Analysis

Datasheet:

The Arima-HIC kit for Reproducible Genome Conformation Analysis

Low-Input Note:

Unlock Low-Input 3D Genome Analysis

Capture HiC Application Note:

Utilizing Agilient SureSelect XT HS2Target Enrichment and Arima-HiC to Improve Resolution of High Throughput Chromatin Conformation Capture