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“We are pleased with the informative nature and quality of the Arima-HiChIP data.  Not only do we observe intrachromosomal cis-regulatory interactions across cancer genomes, but also interchromosomal ectopic cis-regulatory interactions on rearranged chromosomes.  We think this data will help better delineate the relationship between genetic and epigenetic driver events in cancer.”

– Peter Scacheri, PhD, Professor, Case Western University School of Medicine

“We have been very pleased with the performance of the Arima Hi-C/HiChIP kit. It has improved reproducibility and overall quality while saving us time and sequencing cost. We particularly value the straightforward quality control steps and the ease of use. The Arima Technical Support Team has also been outstanding. Overall, a great value.”

– Tamas Ordog, MD, Professor, Mayo Clinic

“We have been homebrewing HiChIP because it provides critical insights that were previously obtained from performing separate Hi-C and ChIP-seq experiments. Arima-HiChIP generated superior data quality and reproducibility even with our most challenging, terminally differentiated, neuronal samples, where our homebrew version previously failed, thus saving us from substantial repeat costs.”

– Yin Shen, PhD, Principal Investigator, University of California, San Francisco

“Enriching for active chromatin features through HiChIP with H3K27ac or H3K4me3 enables our discovery of high-resolution chromatin looping events that facilitate gene control in muscle stem cells during the transition from a state of quiescence to their commitment toward differentiation into regenerating myofibers, during muscle regeneration in healthy conditions, aging, or diseases such as muscular dystrophies. The combined analysis of this HiChIP data with our genome-wide Hi-C data is pivotal to our understanding of not only the broader compartmentalization and topological frameworks that influence gene regulation, but also the finer resolution connections between specific cis-regulatory elements.”

– Pier Lorenzo Puri, MD, PhD, Professor, Sanford Burnham Prebys Discovery Institute

“We have been very happy with the Arima-HiC kit. The Arima-HiC protocol is days quicker than our previous lab’s protocol. Altogether, better quality data for less effort.”

– Eileen Furlong, PhD, Head of Genome Biology Unit – European Molecular Biology Laboratory (EMBL)

“The ability of Arima-HiC kits to deliver greater insight with less sequencing cost will be critical in enabling more researchers to leverage Hi-C technology for understanding gene regulation within the three-dimensional context of the genome.”

– Changhoon Kim, PhD, Chief Technology Officer, Macrogen, Inc.

“Arima Genomics is an integral technology partner of the G10K consortium and Phase I of the VGP project. Arima was selected for Phase I based on the quality of their data, proven by their ability to generate reproducible and high-quality data despite variability in input sample quality and quantity. The long-range genomic interactions from Arima-HiC data is an essential component of our current strategy for the generation of chromosome-spanning reference assemblies.”

– Gene Myers, PhD, G10K Council Member and Director, Max-Planck Institute of Molecular Cell Biology and Genetics

“The Arima HiC kit has worked well in our testing and provided excellent quality genome scaffolds at extremely low sequencing depths. The Arima support team have been very knowledgeable and extremely quick to respond.”

– Phil Ewels, Head of the Genomics Applications Development, SciLifeLab

“When we tested Arima-HiC kit in our hands, we consistently got very high-quality libraries. Particularly, the fraction of interchromosomal reads is reduced.”

– Daan Noordermeer, PhD, Group Leader, Chromatin Dynamics Group, French National Centre for Scientific Research (CNRS)

“We can get a good Hi-C library with sufficient coverage from sequencing one HiSeq lane. With our previous methods we needed three or more lanes to get sufficient coverage.”

– Melissa Fullwood, PhD, Assistant Professor, Nanyang Technical University

“We are pleased with the ease of the Arima-HiC workflow and the improved data quality for topological domain and chromatin looping analysis. The expanded support for low input is a significant feature, as it allows researchers to perform Hi-C across precious samples such as rare cell populations or clinical samples that were previously precluded from Hi-C analysis.”

– Hiruy Meharena, PhD, Postdoctoral Fellow at Massachusetts Institute of Technology

“I really thought it was quite easier compared to other Hi-C methods we evaluated, and y'all's QC checks made me feel a lot better about the library preparation throughout the process.”

– Caitlin Castanada, Texas A&M

“Although my undergraduate researcher, Bianca Cordazzo, a Harvard sophomore, is a motivated quick learner and had a little bit of experience in general lab practice before joining my lab, she was a novice technician of molecular biology. I would not have asked her to construct Hi-C libraries if the Arima-HiC kit were not available. Bianca found this kit easy to learn, and it actually worked in her hands. Bianca analyzed data obtained from the Hi-C libraries constructed by herself, and both she and I were delighted to find clear and reproducible TAD boundaries displayed on our computer screen.”

– Toshi Shioda, MD, PhD, Primary Investigator, Massachusetts General Hospital Center for Cancer Research and Associate Professor of Medicine, Harvard Medical School

 

Arima Genomics: Celebrating Scientists