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Enabling 3D Genomic Discoveries

Without HiChIP loops, traditional immunoprecipitation techniques are limited to the analysis of sequencing depth of coverage alone. HiChIP gives a 3D view of your sequence data, associating peaks in sequencing depth with their distal regulatory partners. This information indicates the spatial organization of your most important sequences, as mediated by proteins like H3K4me3 and CTCF, shown below.

Arima Genomics is an industry-leading provider of Hi-C and HiChIP sample preparation kits. In celebration of this emerging technology, Arima is offering research grants for projects that focus on HiChIP use in the following research areas:

enabling 3d genomica
 

From ChIP-seq to HiChIP

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) allows researchers to discover the genomic locations of any DNA-binding protein of interest. ChIP-seq can map out the location of protein binding sites across the genome, but it is unable to capture spatial information indicating which distal transcriptional targets are being regulated by the DNA-bound proteins.

HiChIP’s proximity ligation technology directly links these protein binding sites to their distal regulatory targets, providing a more holistic understanding of the complex regulatory networks underlying traits of interest.

This grant will be awarded to research groups looking to expand their methodology away from ChIP-seq and into HiChIP.

 

GWAS with HiChIP

Genome-wide association studies (GWAS) are commonly used to link genomic variants to specific phenotypes like disease. When GWAS point to variation in well-annotated loci such as protein-coding regions, the results can provide invaluable insight.

In practice, however, GWAS often indicate causal variation in distal regulatory sequences that are separated from their transcriptional target by several kilobases, obscuring conclusions. With HiChIP, GWAS researchers can directly associate causal variation in distal regulatory sequences with the transcriptional targets that they regulate.

This grant will be awarded to research groups looking to supplement their GWAS initiatives with HiChIP technology.

 

2021 HiChIP Research Grant Application

To apply, submit an abstract not exceeding 500 words outlining your research objectives and motivation.

Successful applicants will receive one free HiC+ Sample Prep Kit ($3k+ value) for use with 8 reactions/samples

 

About Hi-C and HiChIP

Hi-C is a proximity ligation method that captures the three-dimensional structure of chromatin prior to sequencing. By preserving this conformational information, Hi-C enables better studies of regulatory interactions and de novo genome scaffolding.

HiChIP (Hi-C Chromatin immunoprecipitation) takes this technology to the next level, capturing chromatin conformation near targeted DNA-binding proteins, providing an unprecedented glimpse at protein-mediated transcriptional regulation.

 

Arima HiChIP workflow

workflow